SPME萃取頭(探針):
產(chǎn)品編號:9117006 DVB/CAR/PDMS三層復合手動探針(50/30 μm, 1 cm)
● 色譜條件
樣品:2 g 茶葉新鮮葉片樣品放到20 ml 頂空瓶中
萃取溫度:40℃;萃取時間:60 min
解吸時間:2 min;解吸溫度:250℃
樣品進樣口:GC
進樣方式:手動
進樣口:250℃分流,分流比:5:1
色譜柱:HP-INNOWax, 30 m × 250 μm × 0.25 μm
程序升溫:起始溫度50℃,保持5 min,以10℃/min 升到240℃,保持10 min
采集模式:全掃描,掃描范圍:30-400 m/z
離子源:230℃,MS 四極桿:150℃
● 檢測結果
茶葉風味物質 SPME-GC/MS 檢測總離子流色譜圖
共檢測到49種有機化合物,其中包含17種醇、3種醛、8種烷烴、2種烯烴、2種芳香烴、1種酸、15種酯、1種酮,各自含量如下表所示。檢測結果表明,6 號化合物乙酸-3-己烯-1-醇酯含量最高,占總量17.313%。
SPME-GC/MS 檢測結果
序號 |
化合物名稱 |
保留時間 |
峰面積百分比總量(%) |
1 |
3-Hexanol(3-己醇) |
7.471 |
0.239 |
2 |
3-Methyl-1-butanol (3-甲基-1-丁醇) |
7.653 |
0.818 |
3 |
2-Hexenal(2-己烯醛) |
7.954 |
10.425 |
4 |
3,7-Dimethyl-1,3,6-octatriene(3,7-二甲基-1,3,6-辛三烯) |
8.995 |
0.202 |
5 |
Acetic acid hexyl ester(乙酸己酯) |
9.188 |
1.478 |
6 |
3-Hexen-1-ol acetate(乙酸-3-己烯-1-醇酯) |
9.954 |
17.313 |
7 |
2-Hexen-1-ol acetate(乙酸-2-己烯-1-醇酯) |
10.222 |
0.882 |
8 |
1-Hexanol(1-己醇) |
10.45 |
4.448 |
9 |
3-Hexen-1-ol(3-己烯-1-醇) |
10.955 |
16.367 |
10 |
3-Hexen-1-ol formate(甲酸-3-己烯-1-醇酯) |
11.105 |
0.099 |
11 |
2-Hexen-1-ol(2-己烯-1-醇) |
11.261 |
4.799 |
12 |
Butanoic acid hexyl ester(丁酸己酯) |
11.715 |
0.19 |
13 |
Methanol- π methyl- π [4-methyl-3-pentenyl] oxirane |
11.882 |
1.892 |
14 |
1-Heptanol(1-庚醇) |
12.03 |
0.892 |
15 |
5-Ethenyltetrahydro-π π 5-trimethyl-2-furanmethanol |
12.291 |
7.616 |
16 |
Butanoic acid-2-hexenyl ester(丁酸-2-己烯基酯) |
12.508 |
1.964 |
17 |
2-Hexenyl valerate(2-己烯戊酸) |
12.609 |
0.447 |
18 |
3-Hexenyl-π methylbutyrate(π甲基丁酸-3-己烯酯) |
12.713 |
0.084 |
19 |
Dodecanal(十二烷) |
12.886 |
0.13 |
20 |
Benzaldehyde(苯甲醛) |
12.972 |
0.25 |
21 |
3,7-Dimethyl-1,6-octadien-3-ol(3,7-二甲基-1,6-辛二烯-3-醇) |
13.322 |
9.771 |
22 |
1-Octanol(1-辛醇) |
13.45 |
0.524 |
23 |
5-Ethyl-2(5H)-furanone(5-甲基-2(5H)-呋喃酮) |
13.911 |
0.447 |
24 |
1-Nonanol(1-壬醇) |
14.748 |
0.555 |
25 |
Hexanoic acid, 3-hexenyl ester,(己酸, 3-己烯基酯) |
14.907 |
2.009 |
26 |
Oxalic acid, 4-chlorophenyl octadecyl ester |
15.008 |
0.809 |
27 |
Hexanoic acid, 2-hexenyl ester(己酸, 2-己烯基酯) |
15.065 |
1.069 |
28 |
Geranyl isovalerate(異戊酸香葉酯) |
15.216 |
0.095 |
29 |
Heptacosane(二十七烷) |
15.38 |
0.136 |
30 |
Trifluoroacetate-3-hexen-1-ol(三氟-3-己烯-1-醇) |
15.604 |
0.626 |
31 |
3,7-Dimethyl-2,6-octadienal (3,7-二甲基-2,6-辛二烯醛) |
15.663 |
0.829 |
32 |
Methyl salicylate(水楊酸甲酯) |
16.134 |
5.582 |
33 |
2,6,10-Trimethyl-tetradecane(2,6,10-三甲基十四烷) |
16.359 |
0.525 |
Ethyl 3-(3-pyridyl)prop-2-enoate(3-(3-吡啶基)丙烯酸乙酯) |
16.436 |
0.276 |
|
35 |
2-Methyl-1-hexadecanol(2-甲基-1-十六醇) |
16.642 |
0.111 |
36 |
3,7-Dimethyl-2,6-octadien-1-ol(3,7-二甲基-2,6-辛二烯-1-醇) |
16.83 |
1.67 |
37 |
Benzyl alcohol(苯甲醇) |
17.091 |
0.415 |
38 |
Heptacosane(二十七烷) |
17.343 |
0.117 |
39 |
Phenylethyl alcohol(苯乙醇) |
17.491 |
0.352 |
40 |
Octadecane(十八烷) |
17.57 |
0.725 |
41 |
[5,9-Dimethyl-1-(3-phenyl-oxiran-2-yl)-deca-4,8-dienylidene]-(2-phenyl-aziridin-1-yl)-amine([5,9-二甲基-1-(3-苯基環(huán)氧乙烷-2-基)-deca-4,8-亞芐基] –(2-苯基-吡啶-1-基)-胺) |
17.903 |
0.145 |
42 |
Valeric acid, 4-tridecyl ester(戊酸, 4-十三烷基酯) |
18.02 |
0.392 |
43 |
[5,9-Dimethyl-1-(3-phenyl-oxiran-2-yl)-deca-4,8-dienylidene]-(2-phenyl-aziridin-1-yl)-amine([5,9-二甲基-1-(3-苯基環(huán)氧乙烷-2-基)-deca-4,8-亞芐基] – (2-苯基-吡啶-1-基)-胺) |
18.322 |
0.155 |
44 |
14-Octadecenal(14-十八烯) |
18.685 |
0.417 |
45 |
2-Methyl-1-hexadecanol(2-甲基-1-十六醇) |
18.95 |
0.278 |
46 |
Heptacosane(二十七烷) |
19.734 |
0.528 |
47 |
2-Bromo-octadecanal(2-溴-十八烷) |
19.901 |
0.118 |
48 |
14-[4-(1,1,3,3-Tetramethylbutyl)phenoxy-3,6,9,12-tetraoxatetradecan-1-ol (14-[4-(1,1,3,3-四甲基丁基)苯氧基-3,6,9,12-四氧雜十四碳烯-1-醇) |
20.695 |
0.257 |
49 |
1,2-Benzenedicarboxylic acid, butyl octyl ester |
23.667 |
0.529 |